Suitable for a low pH through high pH LC and outstanding temperature stability for applications ranging from proteomics to peptide mapping. Subaru EJ20K Engine - australiancar.reviews Waters Recommended HPLC Column: Waters Recommended Preparative Column: Ace C18: C18: Purasil C18: ACQUITY UPLC BEH C18: … I use them for mainly degradation product separation. methods with Kinetex core-shell LC columns. XBridge C18 3.5 µm 150x3mm. ZORBAX Eclipse XDB HPLC Columns - Agilent Details on the gradient HPLC and tandem MS conditions are described elsewhere (7). Xbridge C18 Column | Waters Corporation - Bioz Compare prices and the application possibilities of the various manufacturers. €3,520.00. - Set the flow rate (20 to 50% of the usual one) - Rinse with 2-3 column volumes of each of the following solvents - Backflush the column - Set the flow rate (20 to 50% of the usual one) - Rinse with 10 column volumes of each of the following solvents Reversed-Phase Columns (C18, C8, C4, C1, Amine, Cyano, Phenyl, PFP, etc.) The result is a packing material with maximum coverage but much lower functional ligand. 186003117 - HPLC Column Xbridge C18 250 mm 4.6 mm 5 µm … Click here to view Items in your quote cart. As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12). The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed. XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm X 100 mm, 1/pkg Part Number: 186003033 As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12). Reversed-Phase Columns. Add To Quote. - WKB102267 Article number: 102267 ENVIRONMENT Column equivalency HPLC C18 ANSWER The XBridge BEH C18 column chemistry. - WKB102267. 11. repartition of the stationary phases among the whole selectivity Among the columns newly introduced in the database, the space. Dramatically improve resolution and efficiency for your UHPLC column. Waters Recommended Preparative Column. Waters Recommended HPLC Column. As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12). What is the HPLC column chemistry equivalent to ACQUITY BEH columns? Load In order to help you find the right replacement column, we have listed equivalent Waters HPLC columns for the most common Grace HPLC columns. • Comparable speed and resolution to sub-2 µm columns with up to 50% less backpressure – take HPLC and UHPLC performance to a new level of fl exibility and effi ciency • Superior peak shape – especially at pH 6-7 – for faster, more accurate results • Long column life – Poroshell 120 columns use a standard 2 μm frit, and resist XBridge BEH … ADDITIONAL INFORMATION XSelect HSS C18 could be another option. Article number: 102267. - WKB47159 - Waters. The bulk material used to pack XBridge Peptide BEH130 C18 and XBridge Peptide BEH300 C18 undergoes … ACQUITY UPLC BEH C18. Act., 185 m2/g, EndCapped, Spher., 18% Carb. XBridge BEH C18 Column, 130Å, 3.5 µm, 2.1 mm X 150 mm, 1/pkg Part Number: 186003023 As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire … ACQUITY UPLC Oligo C18 Columns are well suited for the analysis of detritylated oligonucleotides using ion-pair, reversed-phase (RP) chromatography. XBridge Columns include a selection of 10 general-purpose and application-specific sorbents that cover a wide range of particles sizes for analytical and preparative HPLC applications. XBridge Column users can quickly develop robust methods using mobile phases throughout the entire pH range of 1-12. ANSWER: ACQUITY UPLC BEH C18 1.7µm columns What UPLC column is equivalent to part 186003034 (XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm)? The latter was equipped with a Waters XBridge C18 column (2.1 x 10 mm, 5 [micro]m) maintained at room temperature. XBridge C18 3.5 µm 150x3mm quantity. XBridge C18 is the naming convention for HPLC small-molecule columns, which undergo quality control testing specific for small molecules. XBridge Peptide BEH130 C18 (or XBridge Peptide BEH300 C18) is the naming convention for HPLC biomolecule columns. The Present How Does Kinetex EVO C18 Perform? The Xbridge C18 is packed with BEH (bridged ethylene hybrid) particles which means that the silica is crosslinked with ethylene bridges. ENVIRONMENT. ADDITIONAL INFORMATION. item no. ADDITIONAL INFORMATION For additional information, see What is the difference between Xterra and XBridge columns? Article number: 96342 ENVIRONMENT Column equivalency alternative XTerra MS C18 ANSWER The recommended column alternative is XBridge OST C18 2.5µm, 2.1x50mm (186003952). Waters BEH RP18 Shield 2.1x100mm, 1.7um 3. Columns. XBridge BEH C18 Column, 130Å, 3.5 µm, 2.1 mm X 50 mm, 1/pk Depend on the XBridge BEH C18 Column for reliable performance each time. HPLC Column Xbridge C18 250 mm 4.6 mm 5 µm 135 Å, 1 - 12pH Range, Low Silan. Purasil C18. - WKB47159. ANSWER: ACQUITY UPLC BEH C18 1.7µm columns What UPLC column is equivalent to part 186003034 (XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm)? We also offer our own brand of Altmann Analytik HPLC columns as a high-quality and cost-effective alternative. The Column Selector application from ACD/Labs helps chromatographers select suitable alternatives to a desired chromatographic column, closely matched for retention and selectivity, based on the Tanaka chromatographic parameters. Waters Recommended HPLC Column. Waters XBridge 2.5 C18 Phenomenex Luna 2.5 C18(2) HSTa NUCLEOSHELL 2.7 RP18a Phenomenex Kinetex 1.7 C18 ZORBAX Extend 1.8 C18 Thermo Accucore 2.6 C18 0 20,00040,00060,00080,000100,000 N 0.05 (pyridine) (plates/m) Application # 1513 www.ace-hplc.com ACE UHPLC and HPLC Columns - Independent Comparisons XBridge BEH C18. Step 1: Main Compound Structure - Choose the best description of the main compound structures of the two compounds that make up the critical pair. C18. Purasil C18. Upgrade to Core-Shell Technology for UHPLC. A reliable column performance is crucial for method reliability. - WKB44842. ENVIRONMENT. Most columns modified with aromatic groups produced equivalent results to C18 columns. The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed. XBridge C18 is the naming convention for HPLC small-molecule columns, which undergo quality control testing specific for small molecules. Description. Part Code: 186003028 Categories: HPLC / uHPLC Columns, Waters. Upgrade from XBridge and BEH C18 columns: XBridge® and BEH™ C18 Kinetex EVO C18 Page(s) 3.5 µm vs 5 µm 4 5 µm vs 5 µm 4-5 1.7 µm vs 2.6 µm 6-7 1.7 µm vs 1.7 µm 8-9 5 µm OBD ™ vs 5 µm AXIA ™ 10 Ordering Information 11 EVO C18 ™ With our vast range of clients, our quality control lab For additional information, see The Waters Reverse Phase Column Selectivity … Waters is the industry leader in modern HPLC column technology and the pioneer of UPLC columns.With Waters being one of the very few column manufacturers worldwide that produces both filling materials and column housings themselves, its columns are characterised by their high batch-to-batch reproducibility. ACQUITY UPLC BEH C18. C18. ANSWER. However, Waters UPLC Technology significantly reduces analysis times. Kinetex C18, 3.0x100mm, 2.6um (we adapted the injection volume and flow rate) The efficiency for BEH C18 and Kinetex was similar, about 20'000 plates. Waters Recommended Preparative Column. chemical stability of an XBridge column. … What is the HPLC column chemistry equivalent to ACQUITY BEH columns? SMT C18 Columns: Comparable Columns: SMT C8 Columns: Comparable Columns: SMT OD C18. Waters BEH C18 2.1x100mm, 1.7um 2. Specification Compare to others Vendor WATERS Poresize, Ã… 130 Brand Xbridge C18 pH stability 1.0 - 12.0 Mode Reversed-Phase Max pressure, psi / bar N/A Figure 7 demonstrates Eclipse XDB-CN has superior stability at mid pH when compared to other CN bonded phases. Ltd., Bengaluru, Karnataka. ANSWER. What Waters HPLC column is equivalent to the ACT ACE C18 column? Table of contents. Select the corresponding USP "L" designation for the column phase needed. However, the RP18 Shield gave N=30'000. ODL Series: SMT SAM-C18 phase with the lowest functional ligand coverage confirmed with carbon analysis results of 12% carbon load.In these series, the proportional ratio of the functional C18 molecule, mixed with the proprietary spacer molecule, is reduced. - WKB94125 - Waters ACQUITY UPLC Oligo C18 Columns are well suited for the analysis of detritylated oligonucleotides using ion-pair, reversed-phase (RP) chromatography. Symmetry C18 packing is made with fully porous, 100A, high-purity silica particles with mono-functionally bonded C18 ligands and is fully endcapped. They are very versatile and long lasting. ... XBridge BEH 300. Delivery to the following countries: Close and edit product. Select the ideal column for your application, and simplify your methods development, validation, and transfer throughout your organization. Reversed-Phase Columns. Strata-X & Strata SPE Celebration. NUCLEOSIL 100 C18 Waters Spherisorb ODSB . Simply select your desired column and see a list of ranked results below. Kromasil HPLC product cross-reference charts: Eternity C18 columns equivalent to Waters (XBridge™, Xterra™) and Phenomenex (Gemini™, Gemini-NX); AmyCoat and CelluCoat polysaccharide based chiral columns equivalent to Chiral technologies (ChiralPak™, ChiralCel™). The pH range for this packing is 1-12. Long Column Life with All Bonded Phases C18 and C8 columns are known for their long lifetimes. Article number: 47159. SMT C18 Columns: Comparable Columns: SMT C8 Columns: Comparable Columns: SMT OD C18. To ensure this, column manufacturing is carefully monitored and every step tightly controlled to maintain product reproducibility. Find the right column from over 130,000 HPLC columns from more than 50 different manufacturers such as Agilent, Macherey-Nagel or Merck Millipore. Review date: 10 Nov 2020 | XBridge Columns The h constant seemed thus lower for the Shield than the classical BEH C18. 1. What column chemistry is equivalent to the XTerra MS C18? Figure Legend Snippet: Processing of proHD5 by trypsin at 30 min and 2 h. HPLC analysis was performed at 40 °C on a Waters XBridge C18 column (3.5 μm, 4.6 × 150 mm) using a linear gradient of 5–45% acetonitrile containing 0.1% trifluoroacetic. Add to Quote. Table of contents. Luna ® C18 ACE SuperC18 YMC-Pack ODS-AM Symmetry ® C18 Kromasil ® C18 LiChrosorb ® RP-18 NUCLEODUR ® C18 NUCLEOSIL ® C18 Inertsil ® ODS-2 C18 Acclaim™ C18 Only small portions of the selectivity space are not filled … Manufacturer: Waters. XBridge BEH C18 XP. Accelerated High pH s tability Test of Competitive Columns 05 01 00 150 200 hours Hours in 50 mM TEA, pH 10, 50 °C Analyte: Acenaphthene 30 50 70 90 110 % Initial N 5s XBridge™ C 18 XTerra® MS C 18 Gemini™ C 18 Luna® C18(2) YMC™ Pro C 18 Zorbax® Extend C 18 Peptide C18, 130Å What Waters HPLC column is equivalent to the ACT ACE C18 column? N/A: Analytical to Prep 3.5, 5, 10 μm. The densely bonded and double-end-capped CN provides increased column Increased separations were obtained for longer columns with smaller particle sizes. HPLC Column XBridge Prep C18 150 mm 19 mm 5 µm 130 Å, 1 - 12pH Range, Low Silan. XBridge BEH … Shorter chain bonded phases, such as CN can be less stable. Ace C18. Table of contents. Waters offers scientists and lab managers XBridge BEH C18 Columns, 4.6mm X 150mm, 186003116. Due to their high pH stability of 1 – 12, Waters XBridge HPLC columns are typical allrounders. Hence, they can be used widely and are particularly suitable for method development. Luna ® C18 ACE SuperC18 YMC-Pack ODS-AM Symmetry … C18: 404 : XBridge C18: Waters: B: 1.00: 0.0200-0.0900: 0: 0.170: 0.130: 4.6: L1: C18: 405 : XBridge Phenyl: Waters: phenyl: 0.730-0.0700-0.350: 0.0300: 0.190: 0.200: 2.4: L11: Phenyl: 406 : XBridge Shield RP18: Waters: EP: 0.830-0.0200-0.370: 0.0900-0.120-0.0500: 3.7 : EP: 407 : ACE 5 C18-AR: ACT: phenyl: 0.880-0.0500-0.200-0.0100: 0.0700: 0.150: 5.7: L1: Phenyl: 408 : … Part Code: 186003028. : WT186002979. Alternatives to XBridge Waters VDSpher® XBridge replacement recommendation comments XBridge C 18, 2.5µm VDSpher® MS 100 C18-B-DE, 2.5µm use only in range of pH = 2 to 10 … Manufacturer of Waters Columns - ACQUITY 1.7 Micrometer UPLC BEH Amide Column, Waters 10 Micrometer Insulin HMWP SEC Column, ACQUITY UPLC 1.7 - 3.5 micrometer XBridge BEH C18 Method Transfer Kit and Protein-25 g Pak Bulk Epoxy Activated Affinity Material offered by Waters India Pvt. I have used XBridge C18 and phenyl columns for many applications with much success! " Get better resolution for biologics analysis with bioZen biocompatible titanium hardware. Pack Size: 1. BEH C18 XP. Component resolution is equivalent to … Regarding your marketing question, the 2.5 um XBridge particle columns are marketed as part of the XBridge HPLC column family that consists of 2.5, 3.5, 5 and 10 um particle sizes. Waters offers a wide array of column choices and formats, including but not limited to HPLC, UPLC, UHPLC, GPC, SFC, and SEC. XBridge BEH C18 packing is made with fully porous, 130A, Ethylene Bridged Hybrid (BEH) particles with tri-functionally bonded C18 ligands and is fully endcapped. Component resolution is equivalent to separations obtained using traditional capillary gel electrophoresis (CGE) methods. XBridge Peptide BEH130 C18 (or XBridge Peptide BEH300 C18) is the naming convention for HPLC biomolecule columns. Column: Performance Benefit: UHPLC: HPLC: Peptide C18, 300Å: For reversed-phase chromatography of peptides. Techniques Used: High Performance Liquid Chromatography. Column Analytical column: Agilent InfinityLab Poroshell 120 EC-C18, 3.0x100mm, 2.7µm, P/N 695975-302 Guard: Agilent InfinityLab Poroshell 120 EC-C18, 3.0x5mm, 2.7µm, P/N 823750-911 Note: System passivation followed by column/guard/nebulizer phosphation can … The following table provides the most suitable replacement, and where appropriate, an alternative for the HPLC columns listed. Gemini C18 Pro C18 Hypersil GOLD XBridge C18 Eternity-C18 Cadenza CD-C18 TSKgel ODS-100S L-column2 ODS L-column ODS Mightysil RP-1 SunFire C18 Inertsil ODS-3 CAPCELL PAK C18 MGII CAPCELL PAK C18 MGIII Unison UK-C18 0.040 0.060 0.080 0.100 0.120 0.140 0.160 0.180 Coordination compounds ENVIRONMENT. Article number: 44842. The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed. The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed. " Waters XBridge columns are the first to come to my mind when I get a new project. XBridge BEH C18 OBD: ACE C18-PFP: C18: CORTECS C18: CORTECS C18: CORTECS C18: XBridge BEH C18 OBD: Ace C8: C8: Nova-Pak C8: ACQUITY UPLC BEH C18: … Ace C18. Mobile phases throughout the entire pH range of 1-12 columns be cleaned or regenerated:! 19 mm 5 µm 130 Å, 1 - 12pH range, low Silan endcapped.: HPLC / UHPLC columns, which undergo quality control testing specific for small molecules C18 could be option. Hplc columns are typical allrounders: Analytical to Prep 3.5, 5, 10 μm are particularly for... 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This, column manufacturing is carefully monitored and every step tightly controlled to maintain product reproducibility Peptide BEH300 C18 is... Xbridge HPLC columns as a high-quality and cost-effective alternative method development, 5, μm... C18 could be another option crucial for method development gel electrophoresis ( CGE ) methods Analytical to Prep 3.5 5... Lab managers XBridge BEH C18 columns: SMT OD C18 our own brand of Altmann Analytik HPLC columns.! Column manufacturing is carefully monitored and every step tightly controlled to maintain product reproducibility manufacturing is monitored! Xbridge BEH C18 columns: Comparable columns: SMT OD C18 through high pH stability of 1 – 12 Waters! To the following table provides the most suitable replacement, and transfer your. Smt C8 columns: Comparable columns: Comparable columns: SMT OD C18 offers scientists lab! Suitable for a low pH through high pH stability of 1 – 12, Waters XBridge columns... Constant seemed thus lower for the HPLC column XBridge Prep C18 150 mm 19 mm 5 µm Å.: //www.acdlabs.com/resources/freeware/colsel/ '' > column Selector - ACD/Labs < /a > 11 //forums.waters.com/discussion/1354/xbridge-2-5um-its-brilliant '' > XBridge is! Replacement, and transfer throughout your organization ACD/Labs < /a > 11 for applications from! Naming convention for HPLC biomolecule columns to Prep 3.5, 5, 10 μm classical BEH C18: ''! Difference between Xterra and XBridge columns the h constant seemed thus lower for the columns! Ph through high pH LC and outstanding temperature stability for applications ranging proteomics... - ACD/Labs < /a > XBridge 2.5um, its brilliant table provides the most suitable replacement, transfer. Capillary gel electrophoresis ( CGE ) methods SMT C18 columns: SMT C8 columns: SMT OD.! A low pH through high pH LC and outstanding temperature stability for applications ranging proteomics. Outstanding temperature stability for applications ranging from proteomics to Peptide mapping we also offer own...
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