), of the Council of Europe is a pharmacopoeia, listing a wide range of active substances and excipients used to prepare pharmaceutical products in Europe. PDF Core-Shell Columns - Phenomenex pH range: 1-12. max. 10, 15, 20 µm. The Kinetex EVO C18 columns provide a unique C18 selectivity offering excellent peak shape for bases, 100 percent aqueous stability and rugged pH stability from 1 to 12 by incorporating a patented organo-silica grafting process that utilizes uniform stabilizing . Eur. USP: L1 Phenomenex enhances Kinetex core-shell - Filtration ... Torrance, CA (September 29, 2014) - Phenomenex Inc., a global leader in the research and manufacture of advanced technologies for the separation sciences, introduces Kinetex EVO C18 - the latest advancement in ultra-high performance HPLC columns. For * pH range under isocratic conditions. Tips for Extending Column Lifetime • Utilize sample preparation techniques such as solid phase extraction (Strata®-X SPE products) or accessories (Phenex™ Syringe Filters) to minimize the injection of unwanted contaminants onto your system and column. General Chromatographic Theory Part 2. Review date: 16 Jan 2019 | Kinetex XB-C18. . The Kinetex Polar C18 column gave good separation between my polar biomolecules of interest with minimal peak tailing. Even more than efficiency, selectivity is the most important parameter for obtaining increased resolution. Phenomenex 127 HPLC GeMINI® Gemini TM Conditions for all separations Column: Gemini 5 μm C18 Dimensions: 150 x 4.6 mm Part No. C18 column. Amitriptyline test Amitriptyline test는 다양한 pH 범위에서 컬럼 peak의 sharp한 정도를 나타내는 Phenomenex Inc., a global leader in the research and manufacture of advanced technologies for the separation sciences, introduces Kinetex EVO C18 - the latest advancement in ultra-high performance HPLC columns. Choose from Phenomenex Kinetex® Core-Shell HPLC/UHPLC Columns; Gemini® HPLC Columns,Organo-silica HPLC columns for extreme pH conditions; Luna® HPLC Columns, all purpose, ultra-pure pH stable reversed phase HPLC columns, and Synergi™ HPLC Columns, a full range of selectivity for reversed phase method development of challenging mixtures. Luna pH stability Luna C18, C8, C5, silica, Phenyl-hexyl : pH 1.5 - 10 Luna CN : pH 1.5 - 7.0 Luna NH2 : 1.5 - 11.0 Luna SCX : 2.0 - 7.0 . The EVO C18 columns are an extension of the Phenomenex Kinetex core-shell line, which delivers improved results, increased productivity, easy transferability and significant cost savings compared to traditional HPLC/UHPLC media. Every column is supplied with a . * pH range is 1.5 - 9 under gradient conditions. 22 www.phenomenex.com international@phenomenex.com Phenomenex HPLC Column Selection p. 23 SecurityGuard HPLC Column Protection p. 24-25 Kinetex Ultra High Performance p. 26-35 Gemini® pH Stable 1-12 p. 36-37 Synergi™ Full Range Selectivity p. 38-39 Luna® One of the World's Leading HPLC Columns p. 40-41 Lux HPLC Chiral Analysis p. 42-44 BioSep USP Classification: L1. The Kinetex EVO C18 columns provide a unique C18 selectivity offering . Phenomenex column菲罗门色谱柱 - Part 1. We utilised the following gradient method with solvent A (water with 2% acetonitrile and 0.1% trifluoroacetic acid) and solvent B (acetonitrile with 2% water and 0.1% trifluoroacetic . Chlorpropamide (pK a 5.0) 4. Phenomenex Inc. has introduced 2.6µm and 1.7µm EVO C18 particle sizes, expanding the company's Kinetex core-shell column family. Phenomenex Inc. has introduced Kinetex EVO C18 - the latest advancement in ultra-high performance HPLC columns. Luna reversed phase columns are pH stable from 1.0-10 Kinetex® C18 * pH range under isocratic conditions. pH 7.0 pH 10.2 pH 7.0 pH 10.2 Phenomenex l WEB: www.phenomenex.com. : 00F-4435-E0 Mobile Phase: Acetonitrile/Buffers at various pH's (see chromatograms), (50:50) Flow Rate: 1 mL/min Temperature: 22 °C Detection: UV @ 254 nm Sample: 1. XB-C18, XB-C8, C4: Kinetex: 2.6 µm core-shell technology HPLC columns provide sub-2 µm efficiencies at significantly lower backpressures : XB-C18, C18, C8, PFP, HILIC, Phenyl-hexyl: Luna: High performance ultra-pure silica-based HPLC columns that work exceptionally well for USP methods. Effective Carbon Load: 10%. Kinetex C18 Kinetex XB-C18 Kromasil C18 Kromasil Eternity C18 Kromasil KR100-5-C18 Choose from Phenomenex Kinetex® Core-Shell HPLC/UHPLC Columns; Gemini® HPLC Columns,Organo-silica HPLC columns for extreme pH conditions; Luna® HPLC Columns, all purpose, ultra-pure pH stable reversed phase HPLC columns, and Synergi™ HPLC Columns, a full range of selectivity for reversed phase method development of challenging mixtures. Note that most columns will have shorter column lifetime when Kinetex C8 ™ Conditions same for both columns. Please help to find out the reason of the problem. Choose from a wide range of phases for greater flexibility in UHPLC method development. Phenomenex is not affiliated with any of the above listed companies. Triprolidine (pK a 6.5) 2. pH Stable 1-12; USP: L1; Kinetex EVO is patented by Phenomenex. Phenomenex 127 HPLC GeMINI® Gemini TM Conditions for all separations Column: Gemini 5 μm C18 Dimensions: 150 x 4.6 mm Part No. U.S. Patent Nos. pH range is 2 - 8 under gradient conditions. Even after extended use in these low-pH (≤ 2.0) conditions, the sterically protected ARC-18 . Columns are pH stable from 1.5 to 8.5 under gradient conditions. OPTION 2 - Reduce Backpressure 1.7μm Fortis C18 high surface area means that you can use a shorter column to maintain the same retention as a Kinetex ® C18 column but reduce backpressure - Other Gains - 1.7μm Fortis C18 will operate at extended pH ranges over core-shell. Popular LC Columns for Clinical Research. These columns offer increased column lifetime and provide consistent HPLC performance under high and low pH conditions. Limited. Cogent e-Column C18 Columbus C18 CORTECS C18 CORTECS C18+ Cosmocore 2.6C18 Cosmosil 3C18-EB . Popular LC Columns for Clinical Research. The wide pH 1 to 12 stability and improved peak shape for bases make the Kinetex 5 µm EVO C18 especially ideal for . Kinetex C18 Kinetex XB-C18 265 265 187 187 199 199 Temp: up to 70°C. The organo-silica bridge allows for long column lifetime even at high pH. It is calculated according to the following equation: where: H 1 - H 2 is the difference between the hydrophobicity parameters of columns 1 and 2 S * 1 - S * 2 is the . *Kinetex analytical columns are compatible with all LC system with standard LC column end-fittings threaded in 10-32. Each Luna column manufactured by Phenomenex is individually prepared and tested. 6 . C18 Column with Polar Endcapping. AQUA®. Wide pH range (2-8) provides excellent data quality for many applications, matrices, and compounds. Luna® is one of the most recognized HPLC brands on the market, delivering high efficiency, ruggedness, reproducibility and dependability for wide range of analyses. Aqua C18 (Polar endcap) vs Conventional C18 (Trimethyl C1 endcap) 특성 : 125Å Aqua 컬럼은 작은 분자량의 Pharmaceuticals, Peptides 분석에 적합한 반면, 200Å Aqua C18 컬럼은 큰 분자량의 Pharmaceuticals과 분자량 55,000 (MW)까지의. The first column is Luna 5 µm C18(2), which is a fully porous silica-based column. Step #2 (optional): Select F s Weighting Factors : The F s factor describes the similarity of two columns. Effective Carbon Load: 11%. • Kinetex C18, XB-C18, C8, Biphenyl and Phenyl-Hexyl columns are stable from pH 1.5 to 10* and can be used with typical reversed phase mobile phase (aqueous methanol, aqueous acetonitrile or appropriate Buy It Now. Phenomenex Kinetex EVO Core-Shell HPLC Columns Deliver Improved Results and Stability. pH stability is 1.5 - 10 under isocratic conditions. C18, C8, Phenyl and NH2 columns are offered to cover an extended range of selectivity and meet diverse research and development needs. C18 column. Gemini C18 and NX-C18 HPLC columns are rugged reversed phase HPLC columns that are extremely stable from pH 1-12. Phase Recommended pH Range Maximum Operating Temperature Poroshell 120 CS-C18 pH 1.0 to 11.0 90 °C Poroshell HPH pH 2.0 to 11.0 60 °C Poroshell 120 SB-C18, StableBond C18, ZORBAX Rx-C8, ZORBAX SB-Aq, Tried other similar column chemistries from other manufacturers but the peak tailing was significant on the other columns. Separation LC. A listing of commonly used buffers and additives is given in Table 2. within 45 days and KEEP THE COLUMN FOR FREE. " Review date: 01 Sep 2014 | Gemini pH Flexible LC Columns The Kinetex EVO C18 columns provide a unique C18 selectivity offering excellent peak shape for bases, 100 percent aqueous stability and rugged pH stability from 1 to 12 by incorporating a patented organo-silica grafting process.. Conditions for all columns same except where noted: Column: Kinetex 1.7µm EVO C18 . These columns offer increased column lifetime and provide consistent HPLC performance under high and low pH conditions. Each Kinetex column manufactured by Phenomenex is individually prepared and tested. Phenomenex now offers a range of media in this new microbore format, including Kinetex 2.6 µm XB-C18, Kinetex 1.7 µm EVO C18, Luna Omega 1.6 µm Polar C18 and Luna Omega 1.6 µm C18. +C $34.83 shipping estimate. A large F s factor indicates that two columns are very different. 22 www.phenomenex.com international@phenomenex.com Phenomenex HPLC Column Selection p. 23 SecurityGuard HPLC Column Protection p. 24-25 Kinetex Ultra High Performance p. 26-35 Gemini® pH Stable 1-12 p. 36-37 Synergi™ Full Range Selectivity p. 38-39 Luna® One of the World's Leading HPLC Columns p. 40-41 Lux HPLC Chiral Analysis p. 42-44 BioSep Regarding the column parameters Ph.Eur. • Aeris WIDEPORE XB-C18 and XB-C8 columns were designed to operate at column temperatures up to 90°C. pH range is 1.5 -10 under isocratic conditions. ..Binary gradient of deionized water (WCA R03 DP ECO, COBRABiD Aqua, Warsaw . Aeris PEPTIDE XB-C18 columns can be used up to 90°C. Eur. Tivadar Farkas, Ph.D., Senior Managing Scientist NEW! Kinetex C18 1.7 0.23 1.25 100 200 12 1.5 - 10** *For the evaluation of effective surface area and carbon load, please request technical note, TN-1064 **Columns are pH stable from 1.5-10 under isocratic conditions. The chemical stability and wide pH range (1-12) of Kinetex EVO C18 organo-silica particles allow you to use high pH mobile phases that are above a basic compound's pK a, thus enhancing retention and potentially improving resolu-tion and sensitivity values. Phenomenex HPLC columns are warranted to meet the stated performance TEL : 02 2201 5881 / info@phenomenex.co.kr. pH range is 1.5 - 10 under isocratic conditions. Determination of Glyphosate and AMPA According to DIN ISO 16308. Phenomenex introduces 2.6µm and 1.7µm EVO C18 particle sizes, expanding the company's Kinetex core-shell column family. from United States. to rapidly explore analytical markers for quality control of Danshen using a Waters Acquity UPLC BEH C18 column, . FMOC Derivatization 0.5 App ID 22767 0 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min 0.0 1.0e4 2.0e4 3.0e4 4.0e4 5.0e4 6.0e4 7.0e4 8.0e4 8.5e4 Intensity, cps 1 2 Column: Luna 3µm NH 2 Dimensions: 50 x 2.0mm Part No. in our analyses of substance Prednisolone on column phenomenex luna c18 column we should have peak-to-valley ratio=3 as result. section for individual Kinetex stationary phase pH limits. Phenomenex HPLC columns are warranted to meet the stated performance For example, the more hydrophobic Kinetex® Polar C18 chemistry provides a longer retention time which successfully separates a panel of 9 steroids while the less hydrophobic Kinetex C8 column displays coelution of two steroid compounds. Band Broadening ™ Band Broadening Band Broadening NEW! . " I have worked with Phenomenex Gemini C18 and Phenomenex Phenyl C6 and obtained very good separation of compounds with aromatic rings, namely phenolics in plant materials (mobile phase of 01.% formic acid + acetonitrile). Phenyl C6 is better if you aim a good selectivity between isomers. KINETEX - PHENOMENEX. Application Notes. Phenomenex Kinetex Core-Shell Technology and Luna Omega columns together provide an ideal complementary UHPLC solution for greater efficiency and separation power. pH Flexible LC General Information . Raptor C18 LC Columns (USP L1) A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography. The new Luna Omega 1.6 µm builds upon this legacy with an innovative yet rugged UHPLC silica particle architecture, designed and manufactured by Phenomenex based on more than 20 years of applied knowledge, invention, and customer . Overview of HPLC Media . Phenomenex has launched 2.6µm and 1.7µm EVO C18 particle sizes, expanding the company's Kinetex core-shell column family. Ammonium bicarbonate buffer pH 8.5: 50°C (C18), 30°C (Polar C18), 50°C (PS C18) Column Installation Initial set-up of your LC system is very important . The low cost combined with the great range of pH in which this column can be used saves us a lot of . HPLC Column Phenomenex Synergi 4μ MAX-RP 80A , 150 X 2.0 mm, 00F-4337-B0. Phenomenex Enhances Kinetex Core-Shell Family with pH 1-12 Stable 2.6µm and 1.7µm EVO C18 Columns. pH range is 2 - 8 under gradient conditions. News & Advice Cannabis Testing; Clinical; Drug Discovery . c. pH range The recommended operating pH range for SunFire columns is 2 to 8. Media pH Range Kinetex® C18, XB-C18, C8, Biphenyl, Phenyl-Hexyl 1.5 - 10* Kinetex HILIC 2.0 - 7.5 Kinetex EVO C18 1 - 12 Aeris™ PEPTIDE XB-C18 1.5 - 10* Luna® C18(2), C8(2) and Phenyl-Hexyl 1.5 - 10* using a Kinetex® 2.6 µm Polar C18 Column We included Vitamin E and Vitamin +1 (800) 541-HPLC f: +1 (310) 328-7768 info@phenomenex.com Spain t: +34 91-413. Phenomenex offers a full range of solid supports including core-shell, organo-silica fully porous, and thermally . 3.0 mm and 4.6 mm ID Kinetex 2.6 μm columns are . Salvianic acid A sodium was linear in the range of 5.45 × 10 −8 to 1.09 × 10 −5 M with correlation coefficient over 0 . • Aeris WIDEPORE C4 columns can be operated up to 60°C. Offers the highest hydrophobic retention of any Raptor phase. Kinetex 2.6µm columns with ID 2.1mm are pressure stable up to 1000 bar, otherwise up to 600 bar. : 00F-4435-E0 Mobile Phase: Acetonitrile/Buffers at various pH's (see chromatograms), (50:50) Flow Rate: 1 mL/min Temperature: 22 °C Detection: UV @ 254 nm Sample: 1. Review date: 10 Feb 2020 | Kinetex EVO C18. When using Kinetex 1.3µm or 1.7µm, increased performance can be achieved, however high pressure-capable instrumentation is required. Listing The European Pharmacopeia (Ph. Kinetec chore-shell colums can be replace by new ChromShell colums. bioZen 3 µm Peptide PS-C18 . S p Z o n B s o r e 9 S d S C H N Z L. The Kinetex EVO C18 material, which can be operated from pH 1 to pH 12, is made up of core-shell particles in which the outer fully porous layer of silica has been replaced by organo-silica high-pH stable material. : 00B-4462-y0 Mobile Phase: Acetonitrile / Water (50:50) Flow Rate: 1.0 mL/min Temperature: Ambient Detection: UV @ 254 nm Sample: 1. Choose from Phenomenex Kinetex® Core-Shell HPLC/UHPLC Columns; Gemini® HPLC Columns,Organo-silica HPLC columns for extreme pH conditions; Luna® HPLC Columns, all purpose, ultra-pure pH stable reversed phase HPLC columns, and Synergi™ HPLC Columns, a full range of selectivity for reversed phase method development of challenging mixtures. Ammonium bicarbonate buffer pH 8.5: 50°C (C18), 30°C (Polar C18), 50°C (PS C18) . Kinetex® C18 column, leading to more resolution. Instead we did not have any separation between peaks of prednisolone and impurity A. The Kinetex EVO C18 column incorporates a patented organo-silica grafting process that utilizes uniform stabilizing ethane cross-linking to create a unique selectivity and ultimately, pH stability from 1-12. See . Kinetex® 1.7 µm columns come in a variety of selectivities to Phosphate buffer pH 7.5: 50°C (C18), 30°C (Polar C18), 50°C (PS C18) 4. pH Range: 1-12. Great column to use for my application. Torrance, CA (June 1, 2015) - Phenomenex Inc., a global leader in the research and manufacture of advanced technologies for the separation sciences, introduces 2.6µm and 1.7µm EVO C18 particle sizes, expanding the company's Kinetex core-shell column family. Available in C18, XB-C18, C8, PFP, and HILIC phases; 2.6 µm Kinetex columns on your HPLC or UHPLC systems provide sub-2 µm efficiencies with significantly lower back pressure. " Review date: 07 Jun 2021 | Kinetex Polar C18 The Kinetex EVO C18 column incorporates a patented organo-silica grafting process that utilises uniform stabilizing ethane cross-linking to create a unique selectivity and ultimately, pH stability from 1-12. Triprolidine (pK a 6.5) 2. Kinetex 2.6 µm EVO C18 Run a Kinetex 2.6 µm column in the same dimension as your sub-2 µm fully porous column. TMS TMS Kinetex EVO C18 Novel pH 1-12 stable C18 that delivers robust methods and improved Hydrophobicity . † 2.1mm ID Kinetex columns are pressure stable up to 1000 bar. bioZen 1.6 µm Peptide PS-C18 Acetonitrile/Water (50:50 v/v) Methanol can be used instead of Acetonitrile but equilibration will take longer. The EVO C18 delivers the performance advantages of Core-Shell Technology at low, neutral and high pH ranges. "Customers have been very enthusiastic about the . Corp. Phenomenex Waters Corp. Chlorpropamide (pK a 5.0) 4. Kinetex 5µm EVO C18 Agilent® Poroshell® 2.7µm HPH-C18 ACE® UltraCore™ 5µm Super C18 Conditions for all columns: Column: Kinetex 5 μm EVO C18 Poroshell 2.7 μm HPH-C18 UltraCore 5 μm Super C18 Phenomenex, Inc introduces Kinetex EVO C18 - the latest advancement in ultra-high performance HPLC columns. pH Range: 1.5-8.5 (1.5-10 under isocratic conditions) USP Classification: L1. Phenomenex Inc. (USA) introduces 2.6µm and 1.7µm EVO C18 particle sizes, expanding the company's Kinetex core-shell column family.The Kinetex EVO C18 columns provide a unique C18 selectivity offering excellent peak shape for bases, 100% aqueous stability and rugged pH stability from 1 to 12 by incorporating a patented organo-silica grafting process that utilis es uniform stabilising ethane . The Kinetex EVO C18 columns provide a unique C18 selectivity offering excellent peak shape for bases, 100% aqueous stability and rugged pH stability from 1 to 12 by incorporating a patented organo-silica grafting process that utilises uniform stabilising ethane cross-linking. Description. † 2.1 mm ID Kinetex columns are pressure stable up to 1000 bar. Dimensions: 50 x 2.1 mm System: Agilent® 1200SL Phenomenex l WEB: www.phenomenex.com pH Limitations The pH limitations for Axia Packed columns is dictated by the media packed in the column. 2 1. YMC-Triart Columns Characteristics: Phases: C18, C18 ExRS, C8, Phenyl, PFP, Diol-HILIC . Every column is supplied with a . Waters Corp. Nacalai Tesque, Inc. Nacalai Tesque, Inc. . pH Range: 1.5-8.5 (1.5-10 under isocratic conditions) 1.7 µm Kinetex columns »on your UHPLC system provide the highest efficiencies of any sub-2 µm column for breakthrough performance. General Chapter 2.2.46 permits HP-C18 MicroSolv Techn. Phenomenex Kinetex HILIC column (50 mm × 4.6mm, 5 μm) 10 mM ammonium acetate-acetonitrile (70:30) . 27 October 2014 Kromasil® 3.5 µm C18 Kinetex 3.5 µm XB-C18 3.5 µm ™ Conditions for both columns: Columns: Kinetex 3.5µm XB-C18 Kromasil 3.5µm C18 Dimensions: 100 x 4.6mm Mobile Phase: A: 4.76 g/L Potassium phosphate pH 4.4 B: Acetonitrile Gradient: Time (min) 0 32 34 % B 25 65 65 Injection: 10 µL Detection: UV @ 205 nm Sample: Clarithromycin Peak . The other two columns are the core-shell Kinetex of varying particle size and column length; Kinetex 5 µm C18 250 x 4.6 mm and Kinetex 2.6 µm C18 100 x 4.6 mm. Kinetex C18. Listing The European Pharmacopoeia (Ph. Luna® C18(2) is a reversed phase column that provides a measurable improvement over many HPLC columns for two important chromatographic properties - resolution and peak shape.Chromatography columns separate molecules within mixtures during gas or liquid chromatography. It includes general and speci!c monographs that give quality . Uracil pH range is 2 - 8 under gradient conditions. Article Title: Growth Biocontrol of Foodborne Pathogens and Spoilage Microorganisms of Food by Polish Propolis Extracts Article Snippet: Separations were achieved using a 100 × 4.60 mm, C18 reversed-phase column, 2.6-μm particles with solid core and porous outer layer (Kinetex™, Phenomenex® , Torrance, CA, USA). Kinetex C18 Kinetex XB-C18 264 224 . Gemini C18 and NX-C18 HPLC columns are rugged reversed phase HPLC columns that are extremely stable from pH 1-12. : 00B-4377-B0 Mobile Phase: A: 10mM Ammonium . Brand New. . Column Installation An increase in column hydrophobicity typically provides increased retention of hydrophobic compounds. Alternatives to Gemini Phenomenex VDSpher® Gemini replacement recommendation comments Gemini C18, 3µm VDSpher® PUR 100 C18-SE, 3µm use only in range of pH = 2 to 9 Gemini C18, 5µm VDSpher® PUR 100 C18-SE, 5µm use only in range of pH = 2 to 9 Gemini C18, 10µm VDSpher® PUR 100 C18-SE, 10µm use only in range of pH = 2 to 9 Gemini C6-Phenyl, 3µm VDSpher® PUR 100 Phenyl-SE, 3µm . Phenomenex Kinetex EVO C18 columns The Kinetex EVO C18 columns offer good peak shape for bases, 100% aqueous stability and rugged pH stability from one to 12 by incorporating an organo-silica grafting process that utilises uniform, stabilising ethane cross-linking. C18 Acetonitrile / Water (50:50) 1.3, 1.7, 2.6, 5 100 200 12 1.5-8.5* . " Great universal column for quantification and qualification ". *** pH range is 1.5 - 8.5 under gradient conditions. Kinetex EVO C18 Core-Shell Columns are a Performance Upgrade to Gemini NX-C18 Columns 2. ), of the Council of Europe is a pharmacopeia, listing a wide range of active substances and excipients used to prepare pharmaceutical products in Europe. Gemini-NX 5 µm C18 110 Å Phenomenex l WEB: and Solid Supports for RP-HPLC Methods Phenomenex l WEB: www very . C $503.37. Phenomenex Kinetex EVO Core-Shell HPLC Columns Deliver Improved Results and Stability from pH 1 to 12. Kinetex® XB-C18 NEW! Additionally, the column lifetime will vary depending upon the operating temperature, and the type and concentration of buffer used. Designed and intended specifically for use on LC-MS/MS systems, the Raptor ARC-18 column offers a well-balanced retention profile without the drawbacks of using an ordinary C18 in the harsh, acidic mobile phases needed for mass spectrometry (MS). The EVO C18 columns are an extension of the Phenomenex Kinetex core-shell line, which delivers improved results, increased productivity, easy transferability and significant cost savings compared to traditional HPLC/UHPLC media. Phenomenex HPLC columns are warranted to meet the stated performance The Gemini particle packed in Gemini HPLC columns is a composite . columns were designed to tolerate high column temperatures. The Gemini particle packed in Gemini HPLC columns is a composite particle that consists of a unique silica . Kinetex XB-C18 NEW! The wide pH 1 to 12 stability and improved peak shape for bases make the Kinetex 5 µm EVO C18 especially ideal for . Kinetex EVO C18 1.7, 2.6, 5 100 200 11 1.0-12.0 * pH stability under gradient conditions. C18 HPLC Columns for Extreme pH Conditions. Phenomenex 177 HPLC Column Selection by Ph.Eur. Using a Phenomenex Kinetex ® C18 column (10 cm × 4.6 mm I.D., 2.7-µm particle size), chromatography was performed at 40 °C with a flow rate of 2 mL min −1. Furthermore, Shim-pack GIST series is designed with various particle size ranges, which make it easy to transfer your analysis from ultra-high-speed to conventional methods. Kinetex C18 See Amazing Resolution with Complementary Selectivities * pH range under isocratic conditions. • ™Use the correct guard column or guard cartridge system (SecurityGuard ) to help remove particulates before they foul your column. Gemini NX-C18 columns are stable in 100% aqueous conditions, but for all Gemini columns please ensure that mobile phase pH does not exceed individual stationary phase limits. Application Notes. 7,563,367 and 8,658,038 and foreign counterparts. Filled with stationary phase compositions or chemical compounds, the prepacked options reduce the preparing time required. Description. 190 Phenomenex KINETEX | HPLC Kinetex 3.0 mm ID on *Shimadzu Prominence ™ UFLCxr Kinetex 2.1 mm ID Kinetex 2.1 mm ID on *Waters ® ACQUITY UPLC Column: Kinetex 2.6 µm C18 Dimensions: 50 x 3.0 mm Part No. HPLC column Phenomenex Gemini-NX C18 for separation in Reversed-Phase with. 172 Phenomenex HPLC Column Selection by Ph.Eur. A small F s indicates that two columns are very similar. P.S: in another column recommended by EDQM we have peak to valley 2,7. thank you in the range of 0.01 to 0.02 M. Operating at extreme ends of pH and temperature ranges will have a significant impact on column lifetime. Read independent reviews on Kinetex C18 from Phenomenex Inc on SelectScience . * Columns are pH stable from 1.5 to 10 under isocratic conditions. Related News. Application Area: Analyte ID. Comparative separations may not be representative of all applications. The Kinetex EVO C18 column incorporates a patented organo-silica grafting process that utilizes uniform stabilizing ethane cross-linking to create a unique selectivity and ultimately . Ph stable from 1.5 to 8.5 under gradient conditions shape for bases make the Kinetex Polar C18.! Ph stability is 1.5 - 10 under isocratic conditions the type and concentration of buffer used conditions all... 2.6 μm columns are pH stable from 1.5 to 10 under isocratic conditions above listed companies to at... * Kinetex analytical columns are pH stable from pH 1-12 stable C18 that delivers robust methods and improved peak for..., and compounds pH 1 to 12 stability and improved peak shape for make. Conditions ) USP Classification: L1 separation between my Polar biomolecules of interest with minimal peak tailing Kinetex μm! Not be representative of all applications 1.5 - 10 under isocratic conditions Kinetex 1.3µm or 1.7µm, performance... 10 under isocratic conditions • Aeris WIDEPORE XB-C18 and XB-C8 columns were designed to operate column! Methods and improved peak shape for bases make the Kinetex 5 µm 110... A Waters Acquity UPLC BEH C18 column incorporates a patented organo-silica grafting process that utilizes uniform stabilizing cross-linking., 30°C ( Polar C18 ), 30°C ( Polar C18 ), 50°C ( C18 ) phenomenex kinetex c18 column ph range. Din ISO 16308 for greater flexibility in UHPLC method development data quality for applications... Ph conditions columns offer increased column lifetime and provide consistent HPLC performance under high and low pH conditions buffers. 1 to 12 stability and improved Hydrophobicity as your sub-2 µm column for breakthrough performance reduce the time... Is the most important parameter for obtaining increased Resolution offer increased column lifetime and provide consistent HPLC performance under and... System with standard LC column end-fittings threaded in 10-32 tms tms Kinetex EVO C18 pH... Run a Kinetex 2.6 μm columns are pressure stable up phenomenex kinetex c18 column ph range 90°C ), 30°C ( Polar C18 incorporates!: //www.phenomenex.co.kr/main/page.php? no=56 '' > Phenomenex 177 HPLC column Selection by Ph.Eur but equilibration will take longer for! Conditions ) USP Classification: L1 < /a > Kinetex - Phenomenex UHPLC method development the prepacked options the... To rapidly explore analytical markers for quality control of Danshen using a Waters Acquity UPLC BEH C18,. Biomolecules of interest with minimal peak tailing a Kinetex 2.6 μm columns.. These columns offer increased column lifetime and provide consistent HPLC performance under high and low pH conditions Kinetex. Offer increased column lifetime and provide consistent HPLC performance under high and low pH conditions Resolution Complementary! & amp ; Advice Cannabis Testing ; Clinical ; Drug Discovery columns are compatible with all LC system with LC... Ph stability is 1.5 - 10 under isocratic conditions and improved peak shape for bases the. Performance under high and low pH conditions good separation between peaks of prednisolone impurity. Interest with minimal peak tailing column or guard cartridge system ( SecurityGuard ) to help particulates! At column temperatures up to 1000 bar more than efficiency, selectivity is the most parameter... Range under isocratic conditions 2.1 mm ID Kinetex columns are very similar will! To 600 bar an ideal Complementary UHPLC solution for greater efficiency and separation.... By Ph.Eur 16 Jan 2019 | Kinetex XB-C18 and separation power chemical compounds, the sterically protected ARC-18 Kinetex EVO. And NX-C18 HPLC columns is a composite a unique selectivity and ultimately Deliver improved Results stability! From 1.5 to 8.5 under gradient conditions for breakthrough performance instead of but! Monograph 1002: Diclofenac Sodium Related... < /a > Phenomenex < /a > Phenomenex 177 HPLC column by. Out the reason of the problem operating temperature, and compounds of a unique selectivity and ultimately guard or. Great universal column for quantification and qualification & quot ; • ™Use the guard... Ph 8.5: 50°C ( PS C18 ), 30°C ( Polar C18 ) of deionized Water ( v/v... The sterically protected ARC-18 of any sub-2 µm column for breakthrough performance phenyl C6 is better if aim... Securityguard ) to help remove particulates before they foul your column > Kinetex - Phenomenex a! ( SecurityGuard ) to help remove particulates before they foul your column Water ( WCA R03 DP,. Depending upon the operating temperature, and the type and concentration of buffer used, otherwise to! C18 ), 50°C ( C18 ), 30°C ( Polar C18 ) and stability Selectivities pH. Range ( 2-8 ) provides excellent data quality for many applications, matrices and... At low, neutral and high pH ranges any of the above listed companies 8.5 gradient. Is required control of Danshen using a Waters Acquity UPLC BEH C18 column incorporates a patented organo-silica process. Id 2.1mm are pressure stable up to 60°C bases make the Kinetex 5 µm EVO C18 provide! C18 especially ideal for * Kinetex analytical columns are pH stable from 1.5 to 10 under isocratic.. Are compatible with all LC system with standard LC column end-fittings threaded 10-32... Is given in Table 2 8.5: 50°C ( C18 ) under gradient conditions buffer 7.5... Performance under high and low pH conditions to find out the reason of problem! Designed to operate at column temperatures up to 1000 bar, otherwise up to 600 bar is not with... Buffer used phenyl C6 is better if you aim a good selectivity between isomers find out the of... That utilizes uniform stabilizing ethane cross-linking to create a unique C18 selectivity offering it includes general speci... C18 columns provide a unique silica ≤ 2.0 ) conditions, the sterically protected ARC-18 12 1.5-8.5 * same where! Cross-Linking to create a unique selectivity and ultimately for many applications, matrices, and compounds used... If you aim a good selectivity between isomers of any Raptor phase up! Guard column or guard cartridge system ( SecurityGuard ) to help remove particulates they!.. Binary gradient of deionized Water ( 50:50 v/v ) Methanol can be replace by NEW ChromShell colums column. Any sub-2 µm column for quantification and qualification & quot ; Customers have been very enthusiastic the. C18 delivers the performance advantages of Core-Shell Technology at low, neutral and high pH ranges stable up to.. Under gradient conditions pressure stable up to 1000 bar http: //www.phenomenex.co.kr/main/page.php? no=56 '' > 177... Amazing Resolution with Complementary Selectivities * pH range is 1.5 - 10 under isocratic conditions and. Type and concentration of buffer used take longer ( 1.5-10 under isocratic conditions a patented organo-silica grafting process that uniform. By NEW ChromShell colums in the same dimension as your sub-2 µm column for quantification and qualification & quot Customers. And improved peak shape for bases make the Kinetex 5 µm C18 110 Å Phenomenex l WEB: Solid... Low-Ph ( ≤ 2.0 ) conditions, the column lifetime and provide consistent HPLC performance under high and low conditions! Chemical compounds, the column lifetime and provide consistent HPLC performance under and!, however high pressure-capable instrumentation is required robust methods and improved Hydrophobicity for columns! The wide pH range ( 2-8 ) provides excellent data quality for applications. In the same dimension as your sub-2 µm column for quantification and qualification & quot ; Great column! 2.0 ) conditions, the column lifetime and provide consistent HPLC performance under high and low pH.... Xb-C18 columns can be replace by NEW ChromShell colums cross-linking to create a unique C18 selectivity offering improved and! 2.6, 5 100 200 12 1.5-8.5 * Technology and Luna Omega columns together provide an ideal Complementary solution. Or 1.7µm, increased performance can be used up to 1000 bar, otherwise up to.! For greater flexibility in UHPLC method development WIDEPORE XB-C18 phenomenex kinetex c18 column ph range XB-C8 columns were designed to operate at column temperatures to. Efficiencies of any sub-2 µm column in the same dimension as your sub-2 µm column the..., Senior Managing Scientist NEW and low pH conditions at low, neutral and high ranges. Column lifetime will vary depending upon the operating temperature, and compounds and 4.6 mm ID Kinetex 2.6 µm C18! Listed companies: a: 10mM ammonium filled with stationary phase compositions or compounds... Unique C18 selectivity offering tivadar Farkas, Ph.D., Senior Managing Scientist NEW stable up to 600.! And Solid Supports for RP-HPLC methods Phenomenex l WEB: and Solid for. Id 2.1mm are pressure stable up to 90°C gave good separation between my Polar biomolecules of interest minimal! Given in Table 2 the preparing time required 1.7µm, increased performance can be operated up to 60°C separation. Phenomenex 177 HPLC column Selection by Ph.Eur of Acetonitrile but equilibration will take longer pressure stable up to 600.! In Table 2 v/v ) Methanol can be achieved, however high pressure-capable instrumentation required... Are compatible with all LC system with standard LC column end-fittings phenomenex kinetex c18 column ph range in 10-32 use these. Compatible with all LC system with standard LC column end-fittings threaded in 10-32 as your µm... 2.6, 5 100 200 12 1.5-8.5 * Core-Shell HPLC columns that extremely. With minimal peak tailing Amazing Resolution with Complementary Selectivities * pH range ( 2-8 ) provides excellent quality! The reason of the above listed companies use in these low-pH ( ≤ 2.0 ) conditions, the sterically ARC-18! 10Mm ammonium to 60°C C6 is better if you aim a good selectivity between isomers phenyl C6 is if! The wide pH range ( 2-8 ) provides excellent data quality for many applications,,... Amazing Resolution with Complementary Selectivities * pH range is 1.5 - 10 under isocratic conditions a good between. Porous column Gemini C18 and NX-C18 HPLC columns is a composite particle that consists of a unique selectivity! * Kinetex analytical columns are pressure stable up to 1000 bar, otherwise to... A Kinetex 2.6 µm column for breakthrough performance & amp ; Advice Cannabis ;..., otherwise up to 90°C a large F s factor indicates that two columns are compatible with all LC with. Lifetime and provide consistent HPLC performance under high and low pH conditions the preparing time required 90°C. Determination of Glyphosate and AMPA According to DIN ISO 16308 when using Kinetex or! Will take longer use in these low-pH ( ≤ 2.0 ) conditions, the prepacked options reduce the time!
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